65 and P = 0 84, respectively) But the overall mean change in jo

65 and P = 0.84, respectively). But the overall mean change in joint space was URMC-099 significant

between the two groups (P < 0.0001). Knee joint space of the experimental group narrowed slowly compared to the control group. Therefore, chondroitin sulfate and glucosamine might play a protective role in preserving articular cartilage and provide evidence for therapeutic drugs in adult patients with KBD.”
“Sulfonated-p-cresol (SPC) was used as novel dopant for the first time in the synthesis of polyaniline in 3D nanofiber networks (PANI-3D). Polyaniline in 3D nanofiber network was prepared using organic solvent soluble benzoyl peroxide as oxidizing agent in presence of SPC and sodium lauryl sulfate (SLS) surfactant via inverted emulsion polymerization pathway. The influence of synthesis conditions

such as the concentration of the reactants, stirring/static condition, and temperature etc., on the properties and formation of polyaniline Selleckchem AZD6244 nanofiber network were investigated. Polyaniline in 3D nanofiber network with 40-160 nm (diameter), high yield (134 wt % with respect to aniline used), and reasonably good conductivity (0.1 S/cm) was obtained in 24 h time. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 120: 2421-2427, 2011″
“Background: Triple negative breast cancer (TNBC) is defined as a lack of the expression of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 in breast cancer. Many TNBCs show a profound infiltration of tumor infiltrating Temsirolimus lymphocytes (TILs). It is still uncertain whether these TILs are protumoral or antitumoral. Regulatory T cells (Tregs) play a role in inducing immune tolerance to antigens, and they may be selectively recruited by cancer cells. This study was conducted to evaluate the significance of TILs with an emphasis on forkhead box p3 (Foxp3), which is a marker for CD25+CD4+ Treg in TNBC. Methods: We investigated the Foxp3, CD8 and CD4 expressions in 100 cases of TNBC by immunohistochemistry and using a tissue microarray. The Foxp3 expression was divided as the high and low infiltration

groups (cut-off value=20). Results: The high infiltration group was correlated with higher histologic and nuclear grades. However, Foxp3+ Tregs were decreased in the T3 and T4 TNBCs as compared to that of the T1 and T2 TNBCs. No significant differences were found for the nodal status, lymphovascular invasion, stage, recurrence and overall survival. Conclusions: High Foxp3+ Treg infiltration in TNBC is correlated with the nuclear and histologic grades, but there was no relation to recurrence and overall survival.”
“Previous studies have found differences in rheumatoid hand surgical practice around the world. The specific aim of this study is to compare baseline characteristics of rheumatoid arthritis (RA) patients in the United States (US) and the United Kingdom (UK) that may be influenced by the two different health-care systems.

‘ The median time to detection was at day seven The overall retr

‘ The median time to detection was at day seven. The overall retransplantation rate was 53.1% and was higher in children (61.9%) than in adults (50%, p < 0.03). The overall mortality rate of patients with eHAT was 33.3% (range: 0-80%). Mortality in adults (34.3%) was higher than in children (25%, p < 0.03). The reported risk factors for eHAT were, cytomegalovirus mismatch (seropositive donor liver in seronegative recipient), retransplantation, FRAX597 clinical trial arterial conduits, prolonged operation time, low recipient weight, variant arterial anatomy, and low volume transplantation centers. eHAT is associated

with significant graft loss and mortality. Uniform definitions of eHAT and uniform treatment modalities are obligatory to confirm these

results and to obtain a better understanding of this disastrous complication.”
“Objective The objectives of this study are to determine (i) what daughters, ages 18-24years, of BRCA1/2 mutation carriers understand about their 50% chance of carrying a BRCA1/2 mutation and about risk reduction or management options for mutation carriers, (ii) the extent and nature of daughters’ cancer-related distress, and (iii) the effects of knowing mother’s mutation status on daughters’ future plans. Methods A total of 40 daughters, currently aged 18-24years, of mothers who tested positive for a mutation in BRCA1/2 were invited by mail to participate (with contact information supplied by their mothers). Daughters participated in a qualitative telephone interview about the impact of learning their mother’s mutation status on their understanding of their own cancer risks and their cancer-related distress, and their knowledge of Vorinostat clinical trial screening strategies, risk-reducing surgery, current health status, and future plans. Participants

also completed study-specific demographic and family history questionnaires, the Brief Symptom Inventory-18, Impact of Event Scale (with hereditary https://www.selleck.cn/PD-1-PD-L1.html predisposition to breast/ovarian cancer as the event), and the Breast Cancer Genetic Counseling Knowledge Questionnaire. Results Daughters’ genetic knowledge is suboptimal; gaps and misconceptions were common. Over 1/3 of the daughters reported high cancer-related distress, despite normal levels of general distress. Disclosed genetic information raised future concerns, especially regarding childbearing. Conclusion Targeted professional attention to this high-risk cohort of young women is critical to inform the next generation of daughters of BRCA1/2 mutation carriers and encourage recommended screening by age 25years. Improved uptake of screening and risk reduction options could improve survival, and psychoeducation could reduce cancer-related distress. Copyright (c) 2013 John Wiley & Sons, Ltd.”
“Drugs used for treating inflammatory bowel disease are known to have a number of gastrointestinal and liver adverse effects. 5-ASA products are relatively safe and have few adverse events.

001) In regard to overall survival (OS), the patients

001). In regard to overall survival (OS), the patients RG-7388 molecular weight who had optimal cytoreductive surgery exhibited improved OS results compared to the sub-optimal surgery group (42 vs. 27 months; P < 0.001). However, a Cox model analysis indicated that a greater number of maintenance chemotherapy cycles was a surrogate marker for improved OS (P < 0.001), but surgery type was not (P > 0.05). Duration of overall patient

follow-up exceeds 41 months.

Conclusion In advanced ovarian cancer patients who achieve a CCR following induction chemotherapy, optimal cytoreduction may confer a greater clinical benefit from a maintenance approach compared to suboptimal cytoreduction.”
“In this study, the effect of ultrahigh pressure extraction at pressures of 150 MPa, 250 MPa, 350 MPa and 450 MPa on the total phenolic contents, the extraction yields and the antioxidant

activities of green tea were investigated. The antioxidant activities of these extracts were analyzed using DPPH radical scavenging activity and total antioxidant capacity. The results showed that the phenolic contents and the antioxidant activities of extracts were greatly influenced by high pressure. The total phenolic contents and the antioxidant activities of ultrahigh pressure extraction at 450 MPa were higher than those of other ultrahigh pressure extraction and conventional extraction. The high content of phenolic compounds in the green tea leaves could account for the antioxidant activity. This study indicated that this new technology can benefit the food and pharmaceutical

check details industries. (C) 2011 Elsevier INCB018424 in vivo Ltd. All rights reserved.”
“Objective To explore and compare the differences in the clinicopathological characteristics and prognosis of synchronous primary endometrial and ovarian cancers with primary endometrial cancer metastatic to adnexa.

Materials and methods Between January 1997 and December 2009, 51 cases with endometrial cancer simultaneously with adnexa malignancy were identified. Among them, there were 18 cases with synchronous primary cancers of the endometrium and ovary (Group A) and 33 cases with primary endometrial cancer metastatic to the adnexa (Group B). Clinical and pathologic information was obtained from medical records. Parametric methods were used to compare clinical and pathologic features. Kaplan-Meier survival analysis was performed and compared using log-rank test.

Results The mean age at diagnosis of the disease was 56.6 +/- A 10.8 years (range 34-76 years) in Group A and 53.1 +/- A 9.5 years (range 37-76 years) in Group B. The two groups’ distribution of preoperative image findings, size of endometrial lesion, myometrial invasion, unilateral or bilateral, cervix invasion, and postoperative radiation existed significant differences. With a mean follow-up time of 4.3 +/- A 3.4 years (range 2-11 years), 5-year overall survival (OS) was 75 and 56% in Groups A and B, respectively (p = 0.034).

As both signalling pathways contribute to cancer cell resistance,

As both signalling pathways contribute to cancer cell resistance, ILK seems well suited as a promising tumour target.

Material and methods: Data were obtained by performing a PubMed database search and summarised with a focus on the function of ILK in cancer biology.

Results: The findings on the catalytic function of ILK, on the putative substrates of ILK and on the expression of ILK in tumour and normal tissues are heterogeneous. In the context of cancer, two of these issues might be of importance. First,

a variety of reports indicate a lack of ILK overexpression in tumours. Second, wild-type or overexpression of ILK has been found to considerably PX-478 cell line sensitise tumour cells to ionising irradiation as compared to ILK knockout or ILK knockdown conditions. In contrast, wild-type or overexpression of ILK has been shown to protect tumour cells from chemotherapy-induced cell death.

Conclusions: Due to these conflicting data, it is difficult to evaluate if therapeutic targeting of ILK is a reasonable strategy in cancer therapy. A more comprehensive understanding of the molecular mechanisms controlled by ILK may help to answer this question.”
“Hip fracture is common in the elderly patients with associated high risk of venous thromboembolic complications. Pathogenic activation results in the generation of various Surrogate markers in plasma. This study is designed to identify unique biomarkers in elderly patients

with hip fracture using protein chip array enzyme-linked immunosorbent assay (ELISA) methods. Plasma from a randomized hip fracture study (PK-532; n = 341) treated with either enoxaparin eFT508 concentration (40 mg once daily) or Unfractionated heparin (UFH; learn more 5000 IU twice daily) were collected prior to and at 1, 3, 5, and 7 days. A total of 52 samples were analyzed using proteomic surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry to identify

unique biomarkers in the molecular weight range of 0 to 150 kd. Twenty-nine healthy, volunteer’s and pooled plasma from total hip replacement/total knee replacement patients with a unique biomarker at 11.9 kd were used as quality controls. In the 29 healthy, individuals, the biomarker profile did not reveal the presence of any unique peak in comparison to the reference normal human plasma (NHP). Plasma obtained prior to Surgery exhibits unique biomarkers in 4 of 52 (7.6%) of the samples. On day I postoperatively, 41 of 51 (80.3%) showed a distinct peak at 11.9 kd. On day 3, 43 of 49 (87.8%) patients showed the presence of this biomarker most often at its Strongest intensity. In all, 22 of 44 (50%) showed this biomarker on day 5 and 4 of 23 (17.9%) on day 7. C-reactive protein (CRP), tumor necrosis factor of alpha (TNF-alpha), and serum amyloid A were also increased after Surgery. Tissue factor pathway inhibitor (TFPI) antigen levels were increased due to the treatment modalities.

In addition, the baseline characteristics between donor groups di

In addition, the baseline characteristics between donor groups did not differ except for ovarian morphology. The number of oocytes retrieved and indicators

of embryo quality did not differ between the two groups, and there was no significant difference between pregnancy and miscarriage rates in the recipients.

Oocyte donors with PCO morphology have equivalent pregnancy rates and do not need to be excluded as potential donors.”
“Background: Understanding the epidemiology of death after battlefield injury is vital to combat casualty care performance improvement. The current analysis was undertaken to develop a comprehensive perspective of deaths that occurred after casualties reached a medical treatment facility.

Methods: Battle injury RO4929097 died of wounds (DOW) deaths that occurred after casualties reached a medical treatment facility from October 2001 to June 2009 were evaluated by reviewing autopsy and other postmortem records at the Office of the Armed Forces Medical Examiners (OAFME). A panel of military trauma experts classified the injuries as nonsurvivable (NS) or potentially survivable (PS), in consultation with an OAFME forensic pathologist. Data including demographics, mechanism of injury, physiologic and laboratory variables, and cause of death were obtained from the Joint Theater Trauma Registry and the OAFME selleck chemical Mortality Trauma Registry.

Results: DOW casualties

selleck chemicals (n = 558) accounted for 4.56% of the nonreturn to duty battle injuries over the study period. DOW casualties were classified as NS in 271 (48.6%) cases and PS in 287 (51.4%) cases. Traumatic brain injury was the predominant

injury leading to death in 225 of 271 (83%) NS cases, whereas hemorrhage from major trauma was the predominant mechanism of death in 230 of 287 (80%) PS cases. In the hemorrhage mechanism PS cases, the major body region bleeding focus accounting for mortality were torso (48%), extremity (31%), and junctional (neck, axilla, and groin) (21%). Fifty-one percent of DOW casualties presented in extremis with cardiopulmonary resuscitation upon presentation.

Conclusions: Hemorrhage is a major mechanism of death in PS combat injuries, underscoring the necessity for initiatives to mitigate bleeding, particularly in the prehospital environment.”
“Patients who have suffered burns frequently experience psychological consequences, among which anxiety disorders are prominent. Benzodiazepines are commonly administered to treat these symptoms. The effects of benzodiazepines on healing may not be direct but rather are modulated by alterations of the sleep architecture. This hypothesis is supported by studies that demonstrate the effects of benzodiazepines on the immune system and the inflammatory profile under both normal sleep conditions and during sleep deprivation, particularly rapid eye movement sleep deprivation.

Our results also reveal that during aging the net balance between

Our results also reveal that during aging the net balance between the enzymatic activities involved in 2-AG synthesis and breakdown is low availability of 2-AG in CC membrane fractions and synaptic terminals. Taken together, our results lead us to conclude that these enzymes play crucial roles in the regulation of 2-AG tissue levels during aging. (c) 2012 BioFactors, 2013″
“Objective. Severe pain can lead to ventilatory compromise in patients with multiple

rib fractures. Regional anesthetic techniques, including continuous thoracic paravertebral and thoracic epidural blocks, can be useful in reducing this pain and subsequent morbidity due to respiratory

compromise. ITF2357 Cytoskeletal Signaling inhibitor BKM120 Thoracic paravertebral block can result in significant complications. Presumed epidural spread of injected medication has been described with thoracic paravertebral block. High-quality radiographic images of an attempted placement of a thoracic paravertebral catheter in the epidural space have not been reported. We present these images to highlight the occurrence of this complication.

Settings and Patients. In this case, we report an attempted placement of a thoracic paravertebral catheter that passed into the epidural space. High-fidelity, three-dimensional computer tomography images and the management of the unintended epidural catheterization are presented.

Results and Conclusions. In the setting of severe chest trauma, the potential risk of unintended placement of an intended thoracic paravertebral catheter in the epidural space is graphically illustrated as a potential risk of this procedure.”
“Curcumin (CUR), a yellow pigment in turmeric, CBL-0137 nmr has marked potential for preventing colon cancer. We recently reported that ar-turmerone (ATM) suppressed nitric oxide (NO) generation in macrophages. In the present study, we explored the molecular mechanisms by

which ATM attenuates NO generation and examined the anti-carcinogenesis activity of turmerones (TUR, a mixture of 5 sesquiterpenes including ATM). Both CUR and ATM inhibited lipopolysaccharide (LPS)-induced expression of inducible forms of both nitric oxide synthase and cyclooxygenase (iNOS and COX-2, respectively). A chase experiment using actinomycin D revealed that ATM accelerated the decay of iNOS and COX-2 mRNA, suggesting a post-transcriptional mechanism. ATM prevented LPS-induced translocation of HuR, an AU-rich element-binding protein that determines mRNA stability of certain inflammatory genes. In a colitis model, oral administration of TUR significantly suppressed 2% dextran sulfate sodium (DSS)-induced shortening of the large bowel by 5258%.

7%; p <0 0001) In contrast, failure was least likely to occur

7%; p <0.0001). In contrast, failure was least likely to occur if both the tibial and the femoral component were in a neutral orientation (>= 90 degrees and <8 degrees of valgus, respectively) (failure rate, 0.2% [nine of 4633]; p < 0.0001). “”Correction”" of varus or valgus malalignment of the first implanted component by placement of the second component to attain neutral tibiofemoral alignment was associated with a failure rate of 3.2% (p = 0.4922) for varus tibial malalignment and 7.8% (p = 0.0082) for valgus femoral malalignment. A higher BMI was associated with an increased failure rate. Compared with patients

with a BMI of 23 to 26 kg/m(2), the failure rate in patients with a BMI of >= 41 kg/m(2) increased from 0.7% to 2.6% (p = 0.0046) in well-aligned knees, from 1.6%

to 2.9% (p = 0.0180) in varus knees, and from 1.0% to 7.1% AZD2171 Protein Tyrosine Kinase inhibitor (p = 0.0260) in valgus knees.

Conclusions: Attaining neutrality in all three alignments is important in maximizing total knee implant survival. Substantial “”correction”" of the alignment of one component in order to compensate for malalignment of the other component and thus produce ISRIB a neutrally aligned total knee replacement can increase the risk of failure (p = 0.0082). The use of conventional guides to align a total knee replacement provides acceptable alignment; however, the surgeon should be aware that the patient’s size, as determined by the BMI, is also a major factor in total knee

replacement failure.

Level of Evidence: Prognostic Level II. See Instructions to Authors for a complete description of levels of evidence.”
“The underlying physical mechanism of the so-called colossal dielectric Selumetinib nmr constant phenomenon in CaCu3Ti4O12 (CCTO) thin films were investigated by using semiconductor theories and methods. The semiconductivity of CCTO thin films originated from the acceptor defect at a level similar to 90 meV higher than valence band. Two contact types, metal-semiconductor and metal-insulator-semiconductor junctions, were observed and their barrier heights, and impurity concentrations were theoretically calculated. Accordingly, the Schottky barrier height of metal-semiconductor contact is about 0.8 eV, and the diffusion barrier height of metal-insulator-semiconductor contact is about 0.4-0.7 eV. The defect concentrations of both samples are quite similar, of the magnitude of 10(19) cm(-3), indicating an inherent feature of high defect concentration.”
“We report the simultaneous mapping of multiple histone tail modifications on chromatin that has been confined to nanofluidic channels. In these channels, chromatin is elongated, and histone modification can be detected using fluorescently tagged monoclonal antibodies. Using reconstituted chromatin with three distinct histone sources and two histone tail modification probes (H3K4me3 and H3K9ac), we were able to distinguish chromatin from the different sources.

021) Decreasing total IgG titres were observed in the 0 5-mg gro

021). Decreasing total IgG titres were observed in the 0.5-mg group (p = 0.008), 0.05-mg group (p = 0.004) and 5-mg group (p = 0.078) before and after treatment. For IgG1, IgG2, IgG3 and IgG4 subclasses, in the 0.5-mg group in which the best clinical response was observed, there was statistically significant decreases observed in the IgG2 and IgG3 subclasses (p = 0.047, p = 0.046). Treatment with bCII in patients with RA led to a reduction in anti-collagen II antibody titre indicating an active biological Citarinostat nmr effect as observed previously in animal model of RA. The largest decrease in total and subclasses of anti-collagen antibody titres occurred in the groups of patients with the best therapeutic response

to bCII, supporting the conclusion of the clinical trial and suggests that immune regulation explains the therapeutic effect.”
“We present a theoretical model for mobile ion contamination in a silicon microelectromechanical resonator. In the model both drift and diffusion of the mobile charge in dielectric films are taken into account. The model is verified through a comparison to existing experimental data. We show that the model can describe the frequency drift of resonators in a wide temperature

range. (C) 2011 American Institute of Physics. [doi:10.1063/1.3622511]“
“Metabolic demand for sulfur-containing AA increases during inflammation in nonruminants. Therefore, Met supplementation may alleviate the negative

effects of infection on N balance. Effects of gram-negative PHA-739358 bacterial lipopolysaccharide (LPS) and supplemental dietary Met on N balance, serum hormones and haptoglobin, and plasma urea-N and AA were evaluated in 20 Angus-cross steers (BW = 262 +/- 6.3 kg). Treatments (2 x 2 factorial) were infusion of no LPS (- LPS) or a prolonged low dose of LPS (+ LPS) and dietary supplementation of no (-MET) or 14 g/d (+ MET) of rumen-protected Met (providing 7.9 g/d of DL-Met). Steers were adapted to Sapanisertib molecular weight a roughage-based diet (DMI = 1.4% of BW daily) and supplemental Met for 14 d, and were then infused (1 mL/min via intravenous catheter) with LPS on d 1 (2 mu g/kg of BW) and 3 (1 mu g/kg of BW) of a 5-d collection period. Blood was collected on d 1, before LPS infusion, and at 2, 4, 6, 8, 10, 12, and 24 h after LPS challenge. Diet samples, feed refusals, feces, and urine were collected daily for 5 d. Rectal temperature and serum concentrations of cortisol, prolactin, tumor necrosis factor-alpha, and haptoglobin increased, whereas thyroxine and triiodothyronine decreased for +LPS vs. -LPS steers (LPS x h; P < 0.01). Plasma urea-N was greater for + LPS than -LPS steers (LPS; P = 0.03), and serum IGF-1 was not affected (P >= 0.26) by LPS or Met. Plasma concentrations of Thr, Lys, Leu, Ile, Phe, Trp, Asn, Glu, and Orn decreased, plasma Ala increased, and Gly and Ser initially increased, then declined in + LPS vs. -LPS steers (LPS x h; P = 0.04).

03) For animals that survived 30 minutes and were resuscitated,

03). For animals that survived 30 minutes and were resuscitated, there was no difference in bleeding and/or coagulopathy between groups.

Irrespective of treatment, animals that died after resuscitation demonstrated increased intraperitoneal fluid volume (14.85 mL +/- 1.9 mL vs. 7.02 mL +/- 0.3 mL, p < 0.001).

Conclusion: In this model of mild preinjury hemodilution with plasma, rats that received FFP5 GSK1904529A mouse had decreased survival after uncontrolled hemorrhage from hepatic injury. There were no differences in coagulation function or intraperitoneal fluid volume between the two plasma groups.”
“Background: The occurrence of stroke exhibits a strong circadian pattern with a peak in the morning hours after waking. The factors that influence this pattern of stroke prevalence may confer varying degrees of neuroprotection and therefore influence stroke Entrectinib datasheet severity. This question is difficult to address in clinical cases because of the variability in the location and duration of the ischemic event. Methods: The purpose of this study was to determine if time of day affected

the severity of stroke targeting the motor cortex in rats. Strokes were produced using topical application of the vasoconstrictor endothelin-1 to motor cortex of unanesthetized animals at 2 time points: early day and early night. Behavioral deficits were measured using reaching, cylinder, and horizontal ladder tasks, and the volume of the lesion was quantified. Results: SNX-5422 nmr Behavior on reaching and horizontal ladder tasks were both severely impaired by endothelin-1 treatment compared to vehicle-treated animals, but deficits did not differ according to time of treatment. Similarly, while endothelin-1 produced larger lesions of

the motor cortex than did vehicle treatment, the size of the lesion did not differ according to time of treatment. Conclusions: These results suggest that while many factors under circadian control can influence the prevalence of stroke, the magnitude of lesion and behavioral deficit resulting from an ischemic event may not be influenced by time of day.”
“Background: Pediatric pedestrian injuries are a major health care concern, specifically in urban centers. An educational program (WalkSafe), given one time during the school year, has been shown to improve childhood pedestrian safety. We examined whether this program could create similar long-term cognitive and behavioral changes in our school-aged children.

Methods: An established pediatric pedestrian curriculum was modified slightly for use in our area. Students K-fourth grade were exposed to the program once annually for 2 years. The program was carried out weekly for 3 consecutive weeks. The first and third sessions consisted of an educational module given by the classroom teacher. The second week consisted of an interactive assembly that allowed the children to demonstrate good pedestrian safety using a simulated street.

Taken together, these results demonstrated broad-spectrum antinoc

Taken together, these results demonstrated broad-spectrum antinociceptive properties of cannabinoids in a model of painful diabetic neuropathy. Peripheral activation of both cannabinoid receptors seems to mediate the antinociceptive effect of exogenous or endogenous anandamide. (C) 2012 Elsevier Ltd. All rights reserved.”
“The potential association between psychosis and violence to others has long been debated. Past

research findings are mixed and appear to depend on numerous potential moderators. As such, the authors conducted a quantitative review (meta-analysis) of research on the association between psychosis and violence. A total of 885 effect sizes (odds ratios) were calculated or estimated from 204 studies on the basis of 166 independent data sets. The central tendency (median) of the effect GW4869 chemical structure sizes indicated that psychosis was significantly associated with a 49%-68% increase LXH254 in vivo in the odds of violence. However, there was substantial dispersion among effect sizes. Moderation analyses indicated that the dispersion was attributable in part to methodological factors, such as study design (e.g., community vs. institutional samples), definition and measurement of psychosis (e.g., diagnostic vs. symptom-level measurement, type of symptom), and comparison group (e.g., psychosis compared with externalizing vs. internalizing vs. no mental disorder). The authors discuss these findings in light of potential causal models of the association between psychosis and

violence, the role of psychosis in violence risk assessment and management, and recommendations for future research.”
“We have discovered the first indigenous African hantavirus, Sangassou virus (SANGV). The virus was isolated from an African Torin 1 wood mouse (Hylomyscus simus), trapped in a forest habitat in Guinea, West Africa. Here, we report on the characterization of the genetic and functional properties

of the virus. The complete genome of SANGV was determined and showed typical hantavirus organization. The small (S), medium (M), and large (L) genome segments containing genes encoding nucleocapsid protein, two envelope glycoproteins, and viral polymerase were found to be 1,746, 3,650, and 6,531 nucleotides long, respectively. The exact 5 ‘ and 3 ‘ termini for all three segments of the SANGV genome were determined and were predicted to form the panhandle structures typical of bunyaviruses. Phylogenetic analyses of all three segment sequences confirmed SANGV as a Murinae-associated hantavirus most closely related to the European Dobrava-Belgrade virus. We showed, however, that SANGV uses beta(1) integrin rather than beta(3) integrin and decay-accelerating factor (DAF)/CD55 as an entry receptor. In addition, we demonstrated a strong induction of type III lambda interferon (IFN-lambda) expression in type I IFN-deficient Vero E6 cells by SANGV. These properties are unique within Murinae-associated hantaviruses and make the virus useful in comparative studies focusing on hantavirus pathogenesis.