This tendency remained from days 5 through 14. Development of Na+ transport in epithelial cells of the Reissner’s membrane may contribute considerably to the accomplishment of low Na+ concentration in the endolymph during early neonatal period. NeuroReport 20:1275-1278 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.”
“Bamboo mosaic virus (BaMV) is a 6.4-kb positive-sense RNA virus belonging to the genus Potexvirus of the family Flexiviridae. The 155-kDa viral check details replicase, the product of ORF1, comprises an N-terminal S-adenosyl-L-methionine (AdoMet)-dependent guanylyltransferase,
a nucleoside triphosphatase/RNA 5′-triphosphatase, and a C-terminal RNA-dependent RNA polymerase (RdRp). To search for cellular factors potentially involved in the regulation of replication and/or transcription of BaMV, the viral RdRp domain was targeted as bait to screen against a leaf cDNA library of Nicotiana benthamiana using a yeast two-hybrid system. A putative methyltransferase (PNbMTS1) of 617 amino acid residues without an established physiological function was identified. Cotransfection
of N. benthamiana protoplasts with a BaMV infectious clone and the PNbMTS1-expressing plasmid showed a PNbMTS1 dosage-dependent inhibitory effect on the accumulation of BaMV coat protein. Deletion of the N-terminal 36 amino acids, deletion Birinapant purchase of a predicted signal peptide or transmembrane segment, or mutations in the putative Selleck SC75741 AdoMet-binding motifs of PNbMTS1 abolished the inhibitory effect. In contrast, suppression of PNbMTS1 by virus-induced gene silencing in N. benthamiana increased accumulation of the viral coat protein as well as the viral genomic RNA. Collectively, PNbMTS1 may function as an innate defense protein against the accumulation of BaMV through an uncharacterized mechanism.”
“Neurogenesis persists in certain adult brain regions including the dentate gyrus. Recent studies have shown that long-term potentiation (LTP) induction in the afferent pathway enhances
adult neurogenesis in the dentate gyrus. Here, we investigated whether long-term depression (LTD) induction also affects adult neurogenesis. We induced LTD in the dentate gyrus in one hemisphere and compared the amount of progenitor proliferation with that in the other hemisphere. Unlike LTP induction, LTD induction per se did not affect progenitor cell proliferation in the dentate gyrus. However, when LTD was induced a day before LTP induction, neuronal progenitor cell proliferation facilitated by LTP induction was markedly blunted. These results show that two forms of synaptic plasticity, LTP and LTD, differentially influence hippocampal adult neurogenesis. NeuroReport 20:1279-1283 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.