In addition, uncultured bacteria with 16S rRNA sequences highly similar to members Carfilzomib chemical structure of the Anaerococcus genus have been detected in metagenomes from the human skin flora [6]. A. vaginalis (Li et al. 1992) was first isolated from vaginal discharges and ovarian abscesses [7]. Initially, it was classified in the genus Peptostreptococcus but later reclassified within the genus Anaerococcus [1]. To the best of our knowledge, we first report the isolation of Anaerococcus sp. from the fecal flora of a patient suffering from morbid obesity. Herein, we present a set of features for of A. vaginalis strain PH9 together with the description of the complete genomic sequence and annotation. Classification and features A stool sample was collected from a 26-year-old woman living in Marseille, France, who suffered from morbid obesity: BMI=48.

2 (118.8 kg, 1.57 meter). At the time of stool sample collection, she was not a drug-user and was not on a diet. The patient gave an informed and signed consent, and the agreement of local ethics committee of the IFR48 (Marseille, France) were obtained under agreement 11-017. The fecal specimen was preserved at -80��C after collection. Strain PH9 (Table 1) was isolated in 2011 by anaerobic cultivation on 5% sheep blood-enriched Columbia agar (BioMerieux, Marcy l��Etoile, France) after 4 days of preincubation of the stool sample with addition of thioglycolate in a blood culture bottle. Table 1 Classification and general features of Anaerococcus vaginalis strain PH9 This strain exhibited a 98.8% 16S rRNA sequence similarity with A. vaginalis (Li et al.

1992), the phylogenetically closest validated Anaerococcus species (Figure 1). This value was higher than the 98.7% 16S rRNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species [18]. As a consequence, strain PH9 belongs to A. vaginalis. Figure 1 Phylogenetic tree highlighting the position of Anaerococcus vaginalis strain PH9 relative to other type strains within the Anaerococcus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic … Growth at different temperatures (25, Entinostat 30, 37, 45��C) was tested; no growth occurred at 25��C and 45��C, growth occurred between 30 and 37��C, and optimal growth was observed at 37��C. Colonies were 0.5 mm to 1 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar.