While technology for such interventions is still under development, it is important that computational models spell out their predictions clearly to provide a fundament for definitive testing as soon as the methods are available. Computational models have been particularly important in the search for mechanisms of grid cells. Theoretical models have for example highlighted the potential role of multiple single-cell properties, such as oscillations and after-spike

dynamics, in grid cell formation. With the introduction of in vivo whole-cell patch-clamp and optogenetic methods, the role of these properties can be tested. Direct and controllable manipulation of intrinsic oscillation frequencies, the timing of synaptic http://www.selleckchem.com/products/abt-199.html inputs, or the spiking dynamics of identified grid cells would provide paramount insight into what mechanisms contribute to the formation of spatially responsive neurons. Similarly, network models make strong assumptions about the architecture of

the grid cell circuit, but whether www.selleckchem.com/products/pifithrin-alpha.html the wiring has a Mexican hat pattern or whether connections are circular are examples of questions that cannot be tested until connections between functionally identified neurons can be traced at a large scale. It is possible that a combination of virally based tagging methods and voltage-sensing optical imaging

approaches may get us to this point in the not-too-distant future. Computational models have also offered potential mechanisms for transformation of spatial signals between subsystems of the entorhinal-hippocampal circuit. Current models provide a starting point, for example, for testing hypotheses of how a periodic entorhinal Metalloexopeptidase representation might transform into a nonperiodic hippocampal representation. With emerging technologies such as optogenetics (Yizhar et al., 2011) and virally based tagging (Marshel et al., 2010), it will soon be possible to address the functions of specific inputs to the hippocampus, for example by manipulation of specific spatial wavelengths of the grid signal. New studies will also improve our understanding of interactions that occur within individual brain regions. Anatomical evidence now strongly hints at a modular organization of entorhinal cortical neurons. But what physiological properties or cell types would the anatomical modules correlate with, and how would the individual modules interact to form a cohesive representation of the environment? Existing computational models consider only one or two cell types at most, and none of the current models integrate outputs from border cells, grid cells, and head direction cells.