Traditional NMR spectroscopy operates at Larmor frequencies ��0/2�� neither = �� B0 > 100 MHz yielding �� < 8 ��m for aluminum. This shielding prevents signals from being observed through the 75 ��m thick aluminum layer incorporated into the tote material.A useful food spoilage detector Inhibitors,Modulators,Libraries must have Inhibitors,Modulators,Libraries four essential design features. It should be noninvasive, portable, accept a variety of sample geometries, and probe both metal and non-metal containers. These features are provided by a unilateral NMR circuit, or single-sided coil, which is an NMR tank circuit with a planar inductor that can non-invasively probe a wide range of sample geometries [9]. Interfacing this coil with a light weight permanent magnet that produces a small static magnetic field ultimately leads to increased metal transparency.

The decreased field strength leads to a lower Larmor frequency ��0/2�� �� 5 MHz that concomitantly increases the skin depth in aluminum to �� �� 40 ��m thus providing access Inhibitors,Modulators,Libraries to the study of the contents of non-ferrous, metal-lined containers. In practice, a portable single sided coil and magnet system could be placed flush with a 1,000 L industrial tomato tote to non-invasively provide spoilage information about the contents. Small, portable single sided coil and magnet systems are commercially available with the most well-known example being the NMR Mobile Universal Surface Explorer, developed at RWTH-Aachen [10].The ultimate goal of this work was to determine if NMR spectroscopy is sensitive to tomato paste spoilage and to record the NMR signal from tomato paste in industrial, non-ferrous, metal-lined 1,000 L totes.

To this end, tomato paste NMR relaxation parameters were monitored in small 100 mL glass containers using a customized low magnetic Inhibitors,Modulators,Libraries field NMR spectrometer to find a measureable variable that correlates with tomato spoilage. After the spoilage dependent NMR parameter was determined in non-metal containers, it was confirmed that it can be measured using a sample in a non-ferrous, metal-lined tote using a single sided coil/magnet system. As NMR experiments in metal containers are rarely reported in the literature, effort is spent in the next section describing the ��0/2�� = 4 MHz electromagnet based and the ��0/2�� = 5.25 MHz single sided magnet based systems.2.?Materials and MethodsCommercial tomato paste was used as received from The Morning Star Company.

Small format samples were prepared in a clean room by aseptically transferring fresh tomato paste into thirty 100 mL VWR media bottles. The thirty identical samples were separated Drug_discovery into two, separate, fifteen bottle sets. The control group was set aside while the other fifteen bottles were inoculated with a mixed culture of microorganisms that were harvested from a leaking aseptic filler line at the Los Ba ?os, CA, Morning Star www.selleckchem.com/products/Pazopanib-Hydrochloride.html Company food packing facility. Immediately after preparation, the thirty samples were cooled to T < 5 ��C and stored in a refrigerator.