The CT and TT genotypes were pooled to avoid classes with very small numbers, because only two individuals had the TT genotype (one in the case group and one in the control group). This type of pooling was also used in other studies. Therefore, distinguishing between the dominant or co-dominant model of inheritance for the C and T alleles at this locus and their effect on the studied variables is difficult. However, as expected,

the effect of ethnicity was not observed in the HLA-DR3 /DR4 allele frequency, because these alleles usually confer high susceptibility to T1AD in all populations [4, 5]. The association of C1858T polymorphism with T1AD and other autoimmune diseases was proposed to depend upon the pathogenic LYP-W620 variant that shows increased phosphatase activity and is a gain-of-function inhibitor of T cell signalling www.selleckchem.com/products/Decitabine.html [9]. In our study, this polymorphism was associated with an increased frequency of GAD65 autoantibody and TG autoantibody when the entire cohort (T1AD patients + healthy controls) was considered. Although the T1AD patients had higher frequencies of pancreatic and non-pancreatic autoantibodies than the healthy controls, there

was no association between the *T1858 allele and other islet and organ-specific autoantibodies. Thus, although the frequency of organ-specific autoantibodies in our population 5-Fluoracil was similar to what has been reported previously for Caucasians, this frequency did not depend on the presence of the T1858 allele, except for the autoantibodies against the pancreas and thyroid. The C1858T PTPN22 polymorphism was associated with T1AD susceptibility Thiamet G and autoimmune thyroid disease. Autoantibodies specific to other organs and tissues were frequent in T1AD carriers, predominantly the thyroid glands. The 1858T PTPN22 polymorphism was associated with a higher frequency of GAD65 and TG autoantobody. Allelic variants

in the 5′-proximal region of the IL-21 gene were not related to T1AD susceptibility and other autoimmune diseases. The HLA-DR3 and/or DR4 alleles predominated in T1D patients. We thank Dr George S. Eisenbarth of the Barbara Davis Center for review of the manuscript. We thank Greci S. Paula, Adriana Rosa, Maria de Fátima Sanches and Maria José Pegoraro of the Laboratório de Investigação Médica LIM 18 and to LIM-25, LIM-42, LIM-56 and Hospital das Clínicas da Faculdade de Medicina da USP for technical assistance. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP, process 2006/06390-1. All authors declare they have no conflicting interests. “
“The human homologue of the mouse double minute 2 (MDM2) is known to be overexpressed in a variety of human malignancies. As one of E3 ubiquitin–protein ligases, MDM2 interacts with the tumour suppressor p53 by mediating ubiquitination and degradation of p53.