Subperithecial tissue a dense homogeneous t. epidermoidea–angularis of variously shaped, thin-walled, hyaline cells (5–)7–26(–36) × (4–)5–11(–13) (n = 30); cells smaller towards the base, and interspersed with thick-walled, yellowish hyphae, (2.0–)2.5–4.5(–6.0) μm (n = 30) wide. Asci (75–)88–106(–117) × (4.0–)4.5–5.5(–6.5) μm, stipe (6–)9–23(–35) μm long (n = 73); no croziers seen. Ascospores hyaline, verruculose; cells dimorphic, but often similar; distal cell (3.5–)3.8–5.0(–6.0) × (3.3–)3.5–4.2(–5.0) μm, l/w 1.0–1.3(–1.7) (n = 72), subglobose or slightly elongated and attenuated upward; proximal cell (3.5–)4.3–6.2(–7.6) × (2.7–)3.0–3.6(–4.7) μm, l/w (1.1–)1.3–1.9(–2.3) (n = 72), oblong, wedge-shaped,
#Selleckchem EPZ5676 randurls[1|1|,|CHEM1|]# or subglobose. Cultures and anamorph: optimal growth
at 25°C on all media; no growth at 35°C. On CMD after 72 h 7–11 mm at 15°C, 22–28 mm at 25°C, 11–21 mm at 30°C; mycelium covering plate after 7–8 days at 25°C. Colony hyaline, distinctly circular with well-defined margin, with little mycelium on surface, forming up to 7 broad and 6 narrow concentric zones. Mycelium radially arranged, with conspicuous difference in width between primary and secondary hyphae. Surface hyphae degenerating, appearing empty. Aerial hyphae scant, short, more frequent and longer mainly at distal margin of the plate Autolytic activity and coilings absent or rare. No distinct odour noted. Sometimes pale yellowish on distal margin from 2 weeks, with minute yellow crystals at the very BIBW2992 in vitro distal margin in densely packed mycelium. Chlamydospores (7–)8–12(–16) × (5.5–)6–11(–14) μm, l/w 0.9–1.5(–2) (n = 32), noted after 50 days, uncommon, terminal and intercalary, globose,
ovoid or clavate. Conidiation from 1 to 2 weeks, macroscopically invisible, scant, effuse, on loosely disposed, minute, simple conidiophores spreading from the plug and proximal margin; at distal margin also on long aerial hyphae; greenish only in the stereo microscope; degenerating from ca 3 weeks; cultures usually sterile after several Thymidine kinase transfers. On PDA after 72 h 4–9 mm at 15°C, 19–26 mm at 25°C, 8–14 mm at 30°C; mycelium covering plate after 8–10 days at 25°C. Colonies circular, dense, compact, indistinctly zonate, mycelium radially arranged, surface hyphae becoming moniliform in the centre due to ?chlamydospores. Aerial hyphae inconspicuous, loosely disposed, short and needle-like, superposed by scant thin and long hyphae, decreasing outwards, forming thin radial strands, soon degenerating, collapsing, giving surface finely downy to granular appearance. Autolytic activity and coilings absent or rare. Odour faint, like fermenting fruits (noted from 1 weeks), colony turning pale or greyish yellow, 3AB3–4, 3B5–6, from the centre. Conidiation from 3 to 5 days, macroscopically invisible, effuse, short, spreading from the plug, becoming farinose in the centre, remaining colourless (1 month). At 15°C conidiation dense in white central area.