Reverse micelles are convenient membrane mimetic nanostructures find more and were used here to simulate Cyt c peroxidase activity. Cyt c peroxidase activity was markedly enhanced in sodium dodecyl sulfate (SIDS) reverse micelles. This was dependent on buffer concentration of water pool and mass percentage of buffer in reverse micelles. Fluorescence intensity based on tryptophan residue, electronic absorption curves, and circular dichroism measurements indicated partial unfolding and cleavage of axial methionin 80-Fe bond of Cyt c in reverse micellar medium resulting in the formation of a peroxidase-like artificial enzyme. (C) 2009 Elsevier B.V. All rights reserved.”
“The

essentiality of polyunsaturated lipids makes membranes susceptible to peroxidative modifications. One of the most contemporary examples includes selective peroxidation of cardiolipin in mitochondria of

cells undergoing apoptosis. Cardiolipin www.selleckchem.com/products/Adrucil(Fluorouracil).html peroxidation products are required for the mitochondrial membrane permeabilization, release of pro-apoptotic factors and completion of the cell death program. Therefore, search for effective inhibitors of cardiolipin peroxidation is critical to discovery and development of anti-apoptotic antioxidants. Mitochondria contain significant amounts of a-tocopherol, a well known scavenger of reactive free radicals. In the present study, we used an oxidative lipidomics approach to evaluate the effect of a-tocopherol and its homologues with different lengths of the side-chain such as 2,5,7,8,-tetramethyl-2(4-methylpentyl)-6-chromanol and 2,2,5,7,8-pentamethyl-6-chromanol, on oxidation of tetralinoleoyl cardiolipin induced by cytochrome c in the presence of hydrogen peroxide. Our data indicate that vitamin E homologues inhibit not only accumulation of tetralinoleoyl cardiolipin hydroperoxides but also hydroxy-derivatives of tetralinoleoyl cardiolipin formed in the enzymatic peroxidase half-reaction catalyzed by cytochrome c. Selleck AZD9291 This suggests that protective effects of vitamin E homologues against

tetralinoleoyl cardiolipin peroxidation catalyzed by cytochrome c/hydrogen peroxide are realized largely due to their effects on the peroxidase activity of cytochrome c towards tetralinoleoyl cardiolipin rather than via their scavenging activity.”
“Because the properties and applications of hydrogels are determined by the formation principle and conditions of the hydrogels, novel methods for preparing hydrogels have increasingly triggered scientists’ interest. Here the Diels-Alder reaction was applied to the preparation of hydrogels. For the resultant polymeric diene and dienophile, the Diels-Alder reaction could be performed in water. The gelation time was found to be closely related to the temperature. The gelation time decreased with the temperature increasing.