Moreover, the Expression
of key co-stimulatory and co-regulatory molecules on DC and Treg were examined by flow cytomeric analysis. Results: our findings show that everolimus-treated liver transplant patients maintain a stable DC subset distribution and phenotype. Thus, expression of co-stimulatory and co-regulatory molecule (CD86, CD83, PD-L1 and ICOS-L) did not differ between the 2 groups. Moreover, expression on DC subsets of HLA-DR and human leukocyte antigen (HLA)-G, a non-classical HLA class I molecule, and of its receptor, the immunoglobulin (Ig)-like transcript (ILT)-4 did not Roxadustat clinical trial differ between the mTOR and CNI groups. Notably, however, expression of the ectonucleoti-dase CD39 was significantly higher
on myeloid DC in patients taking everolimus compared with the CNI. Notably, selleck chemical in the ever-olimus group the incidence of Treg was significantly higher and the expression of Programmed death-1 (PD-1) on Treg was significantly lower as compared with CNI group. Conclusions: this preliminary study provides new information about how mTOR inhibitor-based immnosuppression may influence peripheral innate and adaptive immune cells in liver transplant patients. Our findings also point out possible new biomarkers of liver graft acceptance which could be monitored after transplantation. Disclosures: The following people have nothing to disclose: Antonino Castellaneta, Antonio Massaro, María Rendina, Francesca D’Errico, Sonia Carparelli, Angus W. Thomson, Alfredo Di Leo Background Progression of fibrosis after liver transplantation (LT) is associated with a worse outcome. One study suggested that abusive drinking after LT for pure alcoholic cirrhosis was associated selleck chemicals llc with liver fibrosis progression. Aims (1) to evaluate alcohol consumption in France after LT for alcoholic cirrhosis using
carbohydrate deficient transferrin (CDT); (2) To assess fibrosis progression using 2 non-invasive methods. Methods Liver fibrosis progression was assessed using FibroTest (FT, Biopredictive, France) and FibroScan (FS, Echosens, France) in all patients who underwent LT for pure alcoholic cirrhosis in our center, on the same day, once a year. Discordances between the 2 methods were resolved by consensus, after repeating the tests and analysing the files of each patients (clinical examination, biology, ultrasound exam). Excessive drinking was defined by a CDT>1.8%. Parameters of metabolic syndrome were assessed each year. Results Overall, 93 patients were transplanted in La Pitie-Salpetriere (Paris, France) for pure alcoholic cirrhosis, all other causes of hepatopathy being ruled out, between February 2000 and June 2012. Among them, 9 died within the first year after LT, and 9 were not evalu ated (lost of follow-up or residing in a foreign country).