Accumulations of oxidative DNA base lesions (8-oxoG, FapyAde, and FapyGua) were elevated in response to ischemia in both the ipsilateral and contralateral hemispheres, and to a greater extent in the contralateral cortex of OGG1(-/-) mice compared with OGG1(+/+) mice. Ischemia-induced elevation of 8-oxoG incision activity involved increased levels of a nuclear isoform OGG1, suggesting an adaptive response to oxidative nuclear DNA damage. Thus, OGG1 has a pivotal role in repairing oxidative damage to nuclear DNA under ischemic conditions, thereby reducing brain damage and

improving functional outcome. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 680-692; doi:10.1038/jcbfm.2010.147; published online 25 August 2010″
“Objectives: Selleck LDK378 The antibacterial activity of Coffea canephora extract was evaluated in vitro against Streptococcus mutans and Streptococcus sobrinus. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and Blasticidin S time-kill assays. The effect of the extract on dental demineralisation was also investigated.\n\nMethods: Primary 1st molar fragments (n = 24) were inoculated with a saliva pool and sustained in a multiple plaque growth system for 10 days to form biofilm. The biofilm was treated with light roasted C. canephora extract at 20%, Milli-Q water (negative control) and chlorhexidine (positive control) once a day, during a week. Blank controls comprised

fragments without treatment. Biofilm pH was monitored in the last day of treatment. Changes in tooth mineralisation were assessed by cross-sectional 10058-F4 chemical structure microhardness (CSMH) test.\n\nResults: MIC and MBC for S. mutans were 7 +/- 2 mg/mL and 160 +/- 0 mg/mL, respectively, showing no activity for S. sobrinus. The extract produced a 4-log reduction in the number of colonies of S. mutans after 3-h treatment (p < 0.05) with undiluted extract (20%) and MBC concentration (16%). There was no difference among negative/blank controls and coffee plaque pH. Differences between

CSMH values of dental fragments subjected to the coffee extract and to chlorhexidine were not significant. At depths up to 30 mu m from the enamel surface, coffee extract and chlorhexidine promoted higher CSMH values when compared to blank/negative controls (p < 0.05).\n\nConclusion: Our data suggest that light roasted C. canephora extract is beneficial as an anticariogenic substance. (C) 2010 Elsevier Ltd. All rights reserved.”
“Porcine endogenous retroviruses (PERVs) in the pig genome represent a potential risk of infection in pig-to-human transplantation. Long terminal repeats (LTRs) are known to be strong promoter elements that could regulate the transcription activity of PERV elements. It is possible that DNA methylation controls promoter activity of PERV family. Here, we analyzed CpG dinucleotides and CpG islands of six transcribed PERV LTRs. Promoter activity of the LTRs from the six clones methylated by CpG methyltransferase (M.