0 or 9.0) of the dipping polyelectrolyte solutions (PAH and PAA-AgNPs). Figure 6 shows the UV-vis spectra of the LbL-E films as a function of the number of bilayers deposited (10, 20, 30, and 40) at pH 9.0 (solid lines) and only 40 at pH 7.0 (dash line). A better definition in the intensity of the LSPR absorption band around 430 nm is observed when a higher number of bilayers are deposited from 10 to 40, which it is indicative that a higher number of AgNPs are

incorporated. In addition, the LSPR of the AgNPs into the LbL-E films appears at the same wavelength position to that PAA-AgNPs and as a conclusion, no aggregation of AgNPs is observed in the LbL films due to PAA acting as a protective agent and preventing the agglomeration click here Torin 1 clinical trial of the AgNPs during the fabrication process. A study about the thickness evolution of the LbL-E films during the fabrication is performed (see Table 3). As it was expected, an increase of the resultant thickness is observed when the number of bilayers is increased for 10 to 40. Figure 6 UV-vis spectra of the LbL-E thin films

as a function of the number of bilayers. UV-vis spectra of the LbL-E thin films as a function of the number of bilayers from 10 to 40 (solid lines) at pH 9.0 and only 40 at pH 7.0 (dash line). Table 3 Thickness evolution of the thin films obtained LbL-E deposition technique Fabrication process Thickness (nm)

LSPR (λmax; A max) [PAH(9.0)/PAA-AgNPs(9.0)]10 63 ± 5 421.3 nm; 0.017 [PAH(9.0)/PAA-AgNPs(9.0)]20 165 ± 4 432.1 nm; 0.13 [PAH(9.0)/PAA-AgNPs(9.0)]30 507 ± 16 432.3 nm; 0.77 [PAH(9.0)/PAA-AgNPs(9.0)]40 642 ± 12 432.6 nm; 1.18 Thickness evolution of the LbL-E thin films and the Pyruvate dehydrogenase location of the LSPR absorption bands (λmax) with their maxima absorbance values (A max). The influence of the temperature in the LbL-E thin films has been studied. As it was previously performed in the ISS process, the LbL-E films were thermally treated at the same variable temperature values from 50°C to 200°C in order to promote an amide bond cross-link of the polymeric chains. In Figure 7, it is possible to appreciate the evolution of the LSPR absorption band which it remains at the same wavelength position (432.6 nm) from room temperature to the thermal treatment at 150°C. However, a shift in the wavelength position of the LSPR absorption band is observed from 432.6 to 446.9 nm for the higher temperature value (200°C) by forming amide bonds (cross-linked films) with the corresponding partial reduction thickness in comparison with untreated films. In addition, in all the cases of the study, an increase in the maxima absorbance of the LSPR absorption bands is observed after thermal treatment.