The use of a “”control diet”" is fundamentally important to researchers. Two types of “”control diets”" are frequently used in physiological studies: a commercial rodent diet (Purina®) and a diet proposed by the American Institute of Nutrition in 1993 (AIN-93) [12]. However, these two diets have different caloric contents Defactinib (Purina®: 3028.0 Kcal/kg and American Institute of Nutrition diet (AIN-93 M): 3802.7 Kcal/kg) that are not usually considered. Moreover, it is important to determine the JQEZ5 manufacturer possible effects of the feeding protocol and the different diets fed to rodents that are

used in exercise physiology studies. The lactate minimum test has been used in exercise physiology studies of both humans [13–16] and rodents [17–19] because it enables researchers to determine aerobic and anaerobic capabilities in a single test [20, 21]. However, the possible effects of administering diets with different caloric values and feed restriction on the parameters provided by the lactate minimum test are not well understood in the literature. Therefore, the

purpose of this study was to evaluate the effects of dietary restriction (60% of ad libitum intake) of two control diets (commercial Purina® and American Institute of Nutrition diet (AIN-93 M)) on the aerobic and anaerobic capacity of Wistar rats, as determined by the lactate minimum test. Methods Animals and animal care The duration of study was one month and we used 40 Wistar rats that were from 90 days old at the beginning of the click here experiment and had body weights of 406.9 ± 39.44 g. The animals were housed in polyethylene cages measuring 37 × 31 × 16 cm (five rats per cage) at room temperature (25°C) with a 12-hour light/dark photoperiod. All procedures involving animals were submitted to and approved by the Ethics Committee on Animal Use in Research of the Biosciences Institute of UNESP, Rio Claro Campus (protocol number: 2011/6274). The animals were divided into four groups Nabilone with 10 animals per group, depending on the diet and mode of administration. Two groups had access to commercial feed (Purina®): one ad libitum

(ALP) and the other restricted (RAP). The two other groups had access to the diet proposed by the American Institute of Nutrition in 1993 (AIN-93 M): one ad libitum (ALD) and the other restricted (RAD). Feed intake for the animals in the ad libitum groups was recorded daily. Thus, for the animals on feed restriction, feed was offered in an amount corresponding to 60% of the average amount consumed by the ad libitum groups the previous day. This protocol was selected to allow for dietary restriction without causing malnutrition [4]. All groups had free access to water. Diet compositions Commercial Purina ® Diet (Paulínia/SP, Brazil) This diet was composed of 43.7% carbohydrates, 23% protein, and 4% fat at 3,028 kcal/g.